Electrochemistry of Biosystems
Linking Glucose Oxidation to Luminol-based Electrochemiluminescence using bipolar Electrochemistry
Asymmetric Modification of TiO2 Nanofibers with Gold by Electric-Field-Assisted Photochemistry
Stimuli-responsive microgels for electrochemiluminescence amplification

Suraniti E., Vajrala S. R., Goudeau B., Bottari S., Rigoulet M., Devin A., Sojic N., Arbault S.,
Anal. Chem. 2013, 85, 5146-5152.

It is now demonstrated that mitochondria individually function differently because of specific energetic needs in cell compartments but also because of the genetic heterogeneity within the mitochondrial pool-network of a cell. Consequently, understanding mitochondrial functioning at the single organelle level is of high interest for biomedical research, therefore being a target for analyticians. In this context, we developed easy-to-build platforms of milli- to microwells for fluorescence microscopy of single isolated mitochondria. Poly(dimethylsiloxane) (PDMS) was determined to be an excellent material for mitochondrial deposition and observation of their NADH content. Because of NADH autofluorescence, the metabolic status of each mitochondrion was analyzed following addition of a respiratory substrate (stage 2), ethanol herein, and a respiratory inhibitor (stage 3), Antimycin A. Mean levels of mitochondrial NADH were increased by 32% and 62% under stages 2 and 3, respectively. Statistical studies of NADH value distributions evidenced different types of responses, at least three, to ethanol and Antimycin A within the mitochondrial population. In addition, we showed that mitochondrial ability to generate high levels of NADH, that is its metabolic performance, is not correlated either to the initial energetic state or to the respective size of each mitochondrion.


AnalChem2013 p5146 TOC

Potential Induced Fine-tuning the Enantioaffinity of Chiral Metal Phases

Sunday, 16 December 2018
S. Assavapanumat, T. Yutthalekha, P. Garrigue, B. Goudeau, V. Lapeyre, A. Perro, N. Sojic, C. Wattanakit, A. Kuhn Angew. Chem. Int. Ed. (2018) in press Concepts leading to single enantiomers of chiral molecules are of crucial importance for many... Read More...

Surface-confined Electrochemiluminescence Microscopy of Cell Membranes

Wednesday, 14 November 2018
S. Voci, B. Goudeau, G. Valenti, A. Lesch, M. Jović, S. Rapino, F. Paolucci, S. Arbault, N. Sojic J. Am. Chem. Soc., 140, 2018, 14753-14760 Herein is reported a surface-confined microscopy based on electrochemiluminescence (ECL) that allows to... Read More...

Enhanced Annihilation Electrochemiluminescence by Nanofluidic Confinement

Wednesday, 14 November 2018
Hanan Al-Kutubi, Silvia Voci, Liza Rassaei, Neso Sojic, Klaus Mathwig Chem .Sci., 9, 2018, (2018 Chemical Science HOT Article Collection) Microfabricated nanofluidic electrochemical devices offer a highly controlled nanochannel geometry; they... Read More...