Electrochemistry of Biosystems
Linking Glucose Oxidation to Luminol-based Electrochemiluminescence using bipolar Electrochemistry
Asymmetric Modification of TiO2 Nanofibers with Gold by Electric-Field-Assisted Photochemistry
Stimuli-responsive microgels for electrochemiluminescence amplification

Vajrala V. S., Suraniti E., Garrigue P., Goudeau B., Rigoulet M., Devin A., Sojic N., Arbault S.
Anal. Bioanal. Chem. 2014, 406, 931-941. Forefront Paper

Microsystems based on microwell arrays have been widely used for studies on single living cells. In this work, we focused on the subcellular level in order to monitor biological responses directly on individual organelles. Consequently, we developed microwell arrays for the entrapment and fluorescence microscopy of single isolated organelles, mitochondria herein. Highly dense arrays of 3-μm mean diameter wells were obtained by wet chemical etching of optical fiber bundles. Favorable conditions for the stable entrapment of individual mitochondria within a majority of microwells were found. Owing to NADH auto-fluorescence, the metabolic status of each mitochondrion was analyzed at resting state (Stage 1), then following the addition of a respiratory substrate (Stage 2), ethanol herein, and of a respiratory inhibitor (Stage 3), antimycin A. Mean levels of mitochondrial NADH were increased by 29% and 35% under Stages 2 and 3, respectively. We showed that mitochondrial ability to generate higher levels of NADH (i.e., its metabolic performance) is not correlated either to the initial energetic state or to the respective size of each mitochondrion. This study demonstrates that microwell arrays allow metabolic studies on populations of isolated mitochondria with a single organelle resolution.

2014-02

Surface-confined Electrochemiluminescence Microscopy of Cell Membranes


Wednesday, 14 November 2018
S. Voci, B. Goudeau, G. Valenti, A. Lesch, M. Jović, S. Rapino, F. Paolucci, S. Arbault, N. Sojic J. Am. Chem. Soc., 140, 2018, 14753-14760 Herein is reported a surface-confined microscopy based on electrochemiluminescence (ECL) that allows to... Read More...

Enhanced Annihilation Electrochemiluminescence by Nanofluidic Confinement


Wednesday, 14 November 2018
Hanan Al-Kutubi, Silvia Voci, Liza Rassaei, Neso Sojic, Klaus Mathwig Chem .Sci., 9, 2018, (2018 Chemical Science HOT Article Collection) Microfabricated nanofluidic electrochemical devices offer a highly controlled nanochannel geometry; they... Read More...

L’électrochimiluminescence : une méthode de choix pour la bioanalyse


Wednesday, 14 November 2018
L. Bouffier, S. Arbault, A. Kuhn, N. Sojic Techniques de l’Ingénieur. 2018. P156 Les mesures sélectives et sensibles dans des échantillons complexes tels que l’urine ou le sang sont devenues des outils indispensables dans le domaine du... Read More...