Y. Percherancier, B. Goudeau, R. Charlet de Sauvage, F. Poulletier de Gannes, E. Haro, A. Hurtier, N. Sojic, I. Lagroye, S. Arbault, and B. Veyret

Bioelectromagnetics, 2015, 36, 4, 287-293.

The present study focused on gap junctional intercellular communication (GJIC) as a target for biological effects of extremely low-frequency (ELF) magnetic field (MF) exposure. Fluorescence recovery after photobleaching microscopy (FRAP) was used to visualize diffusion of a fluorescent dye between NIH3T3 fibroblasts through gap junctions. The direct effect on GJIC function of a 24 h exposure to 50 Hz MF at 0.4 or 1 mT was assessed in one series of experiments and the potential synergism of MF with an inhibitor of GJIC, phorbol ester (TPA), was studied in another series by observing FRAP when NIH3T3 cells were incubated with TPA for 1 h following a 24 h exposure to MF. In contrast with other reports of ELF-MF effects on GJIC, under our experimental conditions we observed neither direct inhibition of GJIC nor synergism with TPA-induced inhibition by 50 Hz MF exposures.