A. Martin, L. Bouffier, K. B. Grant, B. Limoges, D. Marchal

Analyst, 2016, 141, 4196-4203.

We present a comparative study of ten redox-active probes for use in real-time electrochemical loop-mediated isothermal amplification (LAMP). Our main objectives were to establish the criteria that need to be fulfilled for minimizing some of the current limitations of the technique and to provide future guidelines in the search for ideal redox reporters. To ensure a reliable comparative study, each redox probe was tested under similar conditions using the same LAMP reaction and the same entirely automatized custom-made real-time electrochemical device (designed for electrochemically monitoring in real-time and in parallel up to 48 LAMP samples). Electrochemical melt curve analyses were recorded immediately at the end of each LAMP reaction. Our results show that there are a number of intercalating and non-intercalating redox compounds suitable for real-time electrochemical LAMP and that the best candidates are those able to intercalate strongly into ds-DNA but not too much to avoid inhibition of the LAMP reaction. The strongest intercalating redox probes were finally shown to provide higher LAMP sensitivity, speed, greater signal amplitude, and cleaner-cut DNA melting curves than the non-intercalating molecules.

2016-10

Essential Role of Electrode Materials in Electrochemiluminescence Applications
jeudi 1 décembre 2016
Giovanni Valenti, Andrea Fiorani, Haidong Li, Neso Sojic and Francesco Paolucci ChemElectroChem, 2016, 3, 1990-1997 (selected as spotlights in... Read More...
Combined Local Anodization of Titanium and Scanning Photoelectrochemical Mapping of TiO2 Spot Arrays
mardi 1 novembre 2016
V. Badets, G. Loget, P. Garrigue, N. Sojic,  D. Zigah Electrochimica Acta, 2016, 222, 84–91   Localized growth of TiO2 and rapid screening of TiO2... Read More...
Asymmetric synthesis using chiral encoded metal
mercredi 12 octobre 2016
T. Yutthalekha,C. Wattanakit,V. Lapeyre,S. Nokbin,C. Warakulwit, J. Limtrakul, A. Kuhn  Nature Commun. 2016, 7: 12678 The synthesis of chiral compounds is of crucial importance in many areas of... Read More...
Nanostructured Antimony-Doped Tin Oxide Layers with Tunable Pore Architectures as Versatile Transparent Current Collectors for Biophotovoltaics
mercredi 12 octobre 2016
K. Peters, H. N. Lokupitiya, D. Sarauli, M. Labs, M. Pribil, J. Rathouský, A. Kuhn, D. Leister, M. Stefik, D. Fattakhova-Rohlfing  Adv. Funct. Mater. 2016, 26, 6682–6692 Nanostructured... Read More...
Selective Electrochemiluminescent Sensing of Saccharides using Boronic Acid-Modified Coreactant
mercredi 5 octobre 2016
H. Li, A. C. Sedgwick,  M. Li, R. A. R. Blackburn, S. D. Bull, S. Arbault, T. D. James and N. Sojic Chem. Comm., 2016, in press We report a strategy for modulating the electrogenerated... Read More...
Antagonist Effects Leading to Turn-on Electrochemiluminescence in Thermoresponsive Hydrogel Films
mercredi 5 octobre 2016
H. Li, M. Sentic, V. Ravaine and N. Sojic Phys. Chem. Chem. Phys., 2016, in press We report the effects of the swell-to-collapse transition on the electrochemical and electrochemiluminescent (ECL)... Read More...
Physicochemical and Electronic Properties of Cationic [6]Helicenes, from Chemical and Electrochemical Stabilities to Far-Red (Polarized) Luminescence
mercredi 5 octobre 2016
J. Bosson, G. M. Labrador, S. Pascal, F.-A. Miannay, O. Yushchenko, H. Li, L. Bouffier, N. Sojic, R. C. Tovar, G. Muller, D. Jacquemin, A. D. Laurent, B. Le Guennic, E. Vauthey, J. Lacour Chemistry-A... Read More...
Analytical Electrochemiluminescence
mercredi 5 octobre 2016
H. Cui, F. Paolucci, N. Sojic, G. Xu Analytical and Bioanalytical Chemistry, 2016, 408, 7001–7002
Direct oxidative pathway from amplex red to resorufin revealed by in situ confocal imaging
mardi 4 octobre 2016
P.Lefrançois, V. S. R. Vajrala, I. B. Arredondo, B. Goudeau,Th. Doneux, L. Bouffier,S. Arbault Phys. Chem. Chem. Phys., 2016, 18, 25817-25822. Amplex Red (AR) is a very useful chemical probe... Read More...
Drainage dynamics of thin-liquid films containing soft PNiPAM microgels : influence of the cross-linking density and concentration
lundi 25 juillet 2016
L. Keal, V. Lapeyre, V. Ravaine, V. Schmitt and C. Monteux Soft Matter, 2016, Accepted Manuscript We investigate the drainage dynamics of thin liquid foam films containing PNiPAM microgels... Read More...
Saccharide-induced modulation of photoluminescence lifetime in microgels
lundi 25 juillet 2016
S. A. Denisov, F. Pinaud, M. Chambaud, V. Lapeyre, B. Catargi, N. Sojic, N. D. McClenaghan, V. Ravaine Phys. Chem. Chem. Phys., 2016,18, 16812-16821 Sugar-responsive microgels were prepared by the... Read More...
Templated growth of gold satellites on dimpled silica cores
lundi 18 juillet 2016
C. Chomette, E. Duguet, S. Mornet, V. N. Manoharan, N. B. Schade, C. Hubert, S. Ravaine, A. Perro and M. Treguer-Delapierre Faraday Discussion, 2016. We synthesize robust clusters of gold... Read More...
Elaboration of Metal Organic Framework Hybrid Materials with Hierarchical Porosity by Electrochemical Deposition-Dissolution
samedi 16 juillet 2016
C. Warakulwit, S. Yadnum, C. Boonyuen, C. Wattanakit, A. Karajic, P. Garrigue, N. Mano, D. Bradshaw, J. Limtrakul, A. Kuhn CrystEngComm, 2016,18, 5095-5100 Rationally designed hierarchical... Read More...
Coupling Electrochemistry with Fluorescence Confocal Microscopy To Investigate Electrochemical Reactivity: A Case Study with the Resazurin-Resorufin Fluorogenic Couple
dimanche 5 juin 2016
T. Doneux, L. Bouffier, B. Goudeau, S. Arbault.  Anal. Chem., 2016, 88, 6292-6300.  The redox couple resazurin-resorufin exhibits electrofluorochromic properties which are investigated... Read More...
Regioselective functionalization of dimpled silica particles
lundi 30 mai 2016
C. Hubert,  C. Chomette,  P.-E. Rouet,  A. Désert,  M. Treguer-Delapierre, J. Majimel,  S. Mornet,  A. Perro,  E. Duguet,  S. Ravaine Coll. Surf. A... Read More...
Dual enzymatic detection by bulk electrogenerated chemiluminescence
mardi 24 mai 2016
A. de Poulpiquet, B. Diez-Buitrago, M. Dumont Milutinovic, M. Sentic, S. Arbault, L. Bouffier, A. Kuhn, N. Sojic Anal. Chem., 2016, 88, 6585–6592 The combination of enzymes, as recognition... Read More...
Generation of electrochemiluminescence at bipolar electrodes: concepts and applications
mardi 17 mai 2016
L. Bouffier, S. Arbault, A. Kuhn, N. Sojic Anal. Bioanal. Chem., 2016, 408, 7003–7011 Bipolar electrochemistry (BPE) is an unconventional technique where a conducting object is addressed... Read More...
Real-time electrochemical LAMP: a rational comparative study of different DNA intercalating and non-intercalating redox probes
mardi 17 mai 2016
A. Martin, L. Bouffier, K. B. Grant, B. Limoges, D. Marchal Analyst, 2016, 141, 4196-4203. We present a comparative study of ten redox-active probes for use in real-time electrochemical loop-mediated...
Bright Electrogenerated Chemiluminescence of a Bis-donor Quadrupolar Spirofluorene Dye and its Nanoparticles
mercredi 11 mai 2016
H. Li, J. Daniel, J.-B. Verlhac, M. Blanchard-Desce, N. Sojic Chem. Eur. J., 2016, 22, 12702-12714 A series of symmetric fluorescent dyes built from a spirofluorene core, bearing electroactive... Read More...
Double remote electrochemical addressing and optical readout of electrochemiluminescence at the tip of an optical fiber
mercredi 11 mai 2016
H. Li, P. Garrigue, L. Bouffier, S. Arbault, A. Kuhn, N. Sojic Analyst, 2016, 141, 4299-4304 In this work, we report an original strategy for the wireless electrochemical generation of light at... Read More...
Microscopic imaging and tuning of electrogenerated chemiluminescence with boron-doped diamond nanoelectrode arrays
mercredi 11 mai 2016
M. Sentic, F. Virgilio, A. Zanut, D. Manojlovic, S. Arbault, M. Tormen, N. Sojic, P. Ugo Anal. Bioanal. Chem., 2016, 408, 7085–7094 Nanoelectrode arrays (NEAs) are increasingly applied for a... Read More...
Dual-color electrogenerated chemiluminescence from dispersions of conductive micro-beads addressed by bipolar electrochemistry
mercredi 11 mai 2016
A. de Poulpiquet, B. Diez-Buitrago, M. Milutinovic, B. Goudeau, L. Bouffier, S. Arbault, A. Kuhn, N. Sojic ChemElectroChem, 2016, 3, 404-409 Bipolar electrochemistry (BPE) is exploited here to... Read More...
Patchy colloidal particles for programmed self-assembly
vendredi 8 avril 2016
E. Duguet, C. Hubert, C. Chomette, A. Perro, S. Ravaine   C. R. Chimie. 2016, 19, 173  An increasing theoretical and experimental effort has been recently dedicated to the design and the... Read More...
A Compelling Case for Bipolar Electrochemistry
vendredi 11 mars 2016
A. Kuhn, R. M. Crooks, S. Inagi ChemElectroChem 2016, 3, 351-352 Bipolar electrochemistry is a wireless way of carrying out redox reactions, which is currently undergoing a renaissance highlighted by... Read More...
Electric fields for generating unconventional motion of small objects
jeudi 14 janvier 2016
L. Bouffier, V. Ravaine, N. Sojic and A. Kuhn.  Curr. Opin. Colloid Interface Sci., 2016, 21, 57-64. Electric fields can be used in several ways to generate motion of objects. In this article we... Read More...
Selective Functionalization of Graphene Peripheries by using Bipolar Electrochemistry
lundi 11 janvier 2016
L. Zuccaro, A. Kuhn, M. Konuma, H. Ki Yu, K. Kern, K. Balasubramanian ChemElectroChem, 2016, 3, 372-377. We present a contactless strategy based on bipolar electrochemistry for the local chemical... Read More...
Early Diagnosis of fungal Infections using piezomicrogravimetric and electric Chemosensors based on Polymers molecularly imprinted with D-arabitol
lundi 11 janvier 2016
M. Dabrowski, P. Sindhu Sharma, Z. Iskierko, K. Noworyta, M. Cieplak, S. Oborska, A. Kuhn, W. KutnerBiosensors and Bioelectronics, 2016, 79, 627-635. An elevated concentration of D-arabitol in urine,... Read More...