L. Bouffier, S. Arbault, A. Kuhn, N. Sojic

Anal. Bioanal. Chem., 2016, 408, 7003–7011

Bipolar electrochemistry (BPE) is an unconventional technique where a conducting object is addressed electrochemically in an electrolyte without any wire connection with an external power supply. BPE has been known for decades but remained limited to only a couple of niche applications. However, it is now undergoing a true renewal of interest especially in the context of analytical chemistry. The bipolar electrode exhibits two distinct poles of opposite polarization with respect to the solution. This allows one to separate the localization of sensing elements versus reporting ones. Also, arrays of bipolar microelectrodes can be addressed simultaneously to perform parallel analyses. Among several reporting strategies, the combination of BPE with electro-chemiluminescence (ECL) is the most frequent choice owing to the very simple visual readout provided by ECL. This article reviews the field from the initial reports to the most recent ones, revealing numerous opportunities including novel analytical strategies for the detection of small molecular analytes and biorelevant molecules such as DNA, RNA, peptides, or other biomarkers.



Combining Microfluidics and FT-IR Spectroscopy: Towards spatially resolved information on chemical processes
vendredi 13 octobre 2017
A. Perro, G. Lebourdon, S. Henry, S. Lecomte, L. Servant, S. Marre React. Chem. Eng., 2016,1, 577-594. This review outlines the combination of infrared spectroscopy and continuous microfluidic processes. FTIR spectroscopy gives access to the microscopic chemical composition of samples, which can be correlated with their macroscopic properties. This approach is widely used in chemistry or biology... Read More...
Essential Role of Electrode Materials in Electrochemiluminescence Applications
jeudi 1 décembre 2016
Giovanni Valenti, Andrea Fiorani, Haidong Li, Neso Sojic and Francesco Paolucci ChemElectroChem, 2016, 3, 1990-1997 (selected as spotlights in Angew.​ Chem. Int. Ed.) The phenomenon of electrochemiluminescence (ECL) is luminescence triggered by electrochemical reactions at electrodes. Heterogeneous electron transfers are deeply dependent on... Read More...
Combined Local Anodization of Titanium and Scanning Photoelectrochemical Mapping of TiO2 Spot Arrays
mardi 1 novembre 2016
V. Badets, G. Loget, P. Garrigue, N. Sojic,  D. Zigah Electrochimica Acta, 2016, 222, 84–91   Localized growth of TiO2 and rapid screening of TiO2 photoelectrochemical properties by scanning electrochemical microscopy (SECM) are described. We report the fabrication and operation of an electrochemical tool comprising a microcapillary and an ultramicroelectrode... Read More...
Asymmetric synthesis using chiral encoded metal
mercredi 12 octobre 2016
T. Yutthalekha,C. Wattanakit,V. Lapeyre,S. Nokbin,C. Warakulwit, J. Limtrakul, A. Kuhn  Nature Commun. 2016, 7: 12678 see also CNRS press release The synthesis of chiral compounds is of crucial importance in many areas of society and science, including medicine, biology, chemistry, biotechnology and agriculture. Thus, there is a fundamental interest in developing new approaches for the... Read More...
Nanostructured Antimony-Doped Tin Oxide Layers with Tunable Pore Architectures as Versatile Transparent Current Collectors for Biophotovoltaics
mercredi 12 octobre 2016
K. Peters, H. N. Lokupitiya, D. Sarauli, M. Labs, M. Pribil, J. Rathouský, A. Kuhn, D. Leister, M. Stefik, D. Fattakhova-Rohlfing  Adv. Funct. Mater. 2016, 26, 6682–6692 Nanostructured transparent conducting oxide (TCO) layers gain increasing importance as high surface area electrodes enabling incorporation of functional redox species with high loading. The fabrication of porous TCO... Read More...
Selective Electrochemiluminescent Sensing of Saccharides using Boronic Acid-Modified Coreactant
mercredi 5 octobre 2016
H. Li, A. C. Sedgwick,  M. Li, R. A. R. Blackburn, S. D. Bull, S. Arbault, T. D. James and N. Sojic Chem. Comm., 2016, in press We report a strategy for modulating the electrogenerated chemiluminescence (ECL) response by integrating a boronic acid to the chemical structure of coreactants. Excellent selectivity for D-glucose was achieved by tuning the linker length of a bis-boronic acid amine... Read More...
Antagonist Effects Leading to Turn-on Electrochemiluminescence in Thermoresponsive Hydrogel Films
mercredi 5 octobre 2016
H. Li, M. Sentic, V. Ravaine and N. Sojic Phys. Chem. Chem. Phys., 2016, in press We report the effects of the swell-to-collapse transition on the electrochemical and electrochemiluminescent (ECL) properties of thermoresponsive pNIPAM films incorporating covalently-attached Ru(bpy)32+ luminophores. Upon collapse of the film, the number of electrochemically-active Ru(bpy)32+ centers increases, due... Read More...
Physicochemical and Electronic Properties of Cationic [6]Helicenes, from Chemical and Electrochemical Stabilities to Far-Red (Polarized) Luminescence
mercredi 5 octobre 2016
J. Bosson, G. M. Labrador, S. Pascal, F.-A. Miannay, O. Yushchenko, H. Li, L. Bouffier, N. Sojic, R. C. Tovar, G. Muller, D. Jacquemin, A. D. Laurent, B. Le Guennic, E. Vauthey, J. Lacour Chemistry-A European Journal (selected by the Editor as Front Cover), 2016, in press Physicochemical properties of cationic dioxa 1, azaoxa 2 and diaza 3 [6]helicenes demonstrate a much higher chemical... Read More...
Analytical Electrochemiluminescence
mercredi 5 octobre 2016
H. Cui, F. Paolucci, N. Sojic, G. Xu Analytical and Bioanalytical Chemistry, 2016, 408, 7001–7002
Direct oxidative pathway from amplex red to resorufin revealed by in situ confocal imaging
mardi 4 octobre 2016
P.Lefrançois, V. S. R. Vajrala, I. B. Arredondo, B. Goudeau,Th. Doneux, L. Bouffier,S. Arbault Phys. Chem. Chem. Phys., 2016, 18, 25817-25822. Amplex Red (AR) is a very useful chemical probe that is employed in biochemical assays. In these assays,the non-fluorescent AR is converted to resorufin (RS), which strongly absorbs in the visible region (labs =572 nm) and yields strong fluorescence... Read More...
Drainage dynamics of thin-liquid films containing soft PNiPAM microgels : influence of the cross-linking density and concentration
lundi 25 juillet 2016
L. Keal, V. Lapeyre, V. Ravaine, V. Schmitt and C. Monteux Soft Matter, 2016, Accepted Manuscript We investigate the drainage dynamics of thin liquid foam films containing PNiPAM microgels suspensions with two cross-linking densities (1.5 % or 5% mol BIS) and at two microgel concentrations (0.1 and 1% wt). For this purpose we use a thin film pressure balance apparatus that can apply a controlled... Read More...
Saccharide-induced modulation of photoluminescence lifetime in microgels
lundi 25 juillet 2016
S. A. Denisov, F. Pinaud, M. Chambaud, V. Lapeyre, B. Catargi, N. Sojic, N. D. McClenaghan, V. Ravaine Phys. Chem. Chem. Phys., 2016,18, 16812-16821 Sugar-responsive microgels were prepared by the covalent grafting of a poly(N-isopropylacrylamide) (pNIPAM) matrix with phenylboronic acid (PBA) as a saccharide sensing unit and a [Ru(bpy)3]2+ derivative (2,2′-bipyridine) as a luminescent reporter.... Read More...
Templated growth of gold satellites on dimpled silica cores
lundi 18 juillet 2016
C. Chomette, E. Duguet, S. Mornet, V. N. Manoharan, N. B. Schade, C. Hubert, S. Ravaine, A. Perro and M. Treguer-Delapierre Faraday Discussion, 2016. We synthesize robust clusters of gold satellites positioned with tetrahedral symmetry on the surface of a patchy silica core by adsorption and growth of gold on the patches. First we conduct emulsion polymerization of styrene in the presence of... Read More...
Elaboration of Metal Organic Framework Hybrid Materials with Hierarchical Porosity by Electrochemical Deposition-Dissolution
samedi 16 juillet 2016
C. Warakulwit, S. Yadnum, C. Boonyuen, C. Wattanakit, A. Karajic, P. Garrigue, N. Mano, D. Bradshaw, J. Limtrakul, A. Kuhn CrystEngComm, 2016,18, 5095-5100, COVER Rationally designed hierarchical macro-/microporous HKUST-1 electrodes were prepared via an electrochemical deposition-dissolution technique with the motivation to overcome diffusion limits that are typically occurring for conventional... Read More...
Coupling Electrochemistry with Fluorescence Confocal Microscopy To Investigate Electrochemical Reactivity: A Case Study with the Resazurin-Resorufin Fluorogenic Couple
dimanche 5 juin 2016
T. Doneux, L. Bouffier, B. Goudeau, S. Arbault.  Anal. Chem., 2016, 88, 6292-6300.  The redox couple resazurin-resorufin exhibits electrofluorochromic properties which are investigated herein by absorption and fluorescence spectroelectrochemistry and by electrochemically coupled-fluorescence confocal laser scanning microscopy (EC-CLSM). At pH 10, the highly fluorescent resorufin dye is... Read More...
Regioselective functionalization of dimpled silica particles
lundi 30 mai 2016
C. Hubert,  C. Chomette,  P.-E. Rouet,  A. Désert,  M. Treguer-Delapierre, J. Majimel,  S. Mornet,  A. Perro,  E. Duguet,  S. Ravaine Coll. Surf. A 2016 doi:10.1016/j.colsurfa.2016.05.089 Multipod-like particles made of a silica core surrounded by polystyrene nodules have been used as precursors to synthesize silica particles with 3 or 12 dimples... Read More...
Dual enzymatic detection by bulk electrogenerated chemiluminescence
mardi 24 mai 2016
A. de Poulpiquet, B. Diez-Buitrago, M. Dumont Milutinovic, M. Sentic, S. Arbault, L. Bouffier, A. Kuhn, N. Sojic Anal. Chem., 2016, 88, 6585–6592 The combination of enzymes, as recognition elements for specific analytes, and of electrogenerated chemiluminescence (ECL) as a readout method has proven to be a valuable strategy for sensitive and specific analytical detection. However, ECL is... Read More...
Generation of electrochemiluminescence at bipolar electrodes: concepts and applications
mardi 17 mai 2016
L. Bouffier, S. Arbault, A. Kuhn, N. Sojic Anal. Bioanal. Chem., 2016, 408, 7003–7011 Bipolar electrochemistry (BPE) is an unconventional technique where a conducting object is addressed electrochemically in an electrolyte without any wire connection with an external power supply. BPE has been known for decades but remained limited to only a couple of niche applications. However, it is now...
Real-time electrochemical LAMP: a rational comparative study of different DNA intercalating and non-intercalating redox probes
mardi 17 mai 2016
A. Martin, L. Bouffier, K. B. Grant, B. Limoges, D. Marchal Analyst, 2016, 141, 4196-4203. We present a comparative study of ten redox-active probes for use in real-time electrochemical loop-mediated isothermal amplification (LAMP). Our main objectives were to establish the criteria that need to be fulfilled for minimizing some of the current limitations of the technique and to provide future... Read More...
Bright Electrogenerated Chemiluminescence of a Bis-donor Quadrupolar Spirofluorene Dye and its Nanoparticles
mercredi 11 mai 2016
H. Li, J. Daniel, J.-B. Verlhac, M. Blanchard-Desce, N. Sojic Chem. Eur. J., 2016, 22, 12702-12714 A series of symmetric fluorescent dyes built from a spirofluorene core, bearing electroactive end-groups and having different conjugated linkers were prepared in view of their use as building blocks for the preparation of electrochemiluminescent (ECL) dyes and nanoparticles. Their... Read More...
Double remote electrochemical addressing and optical readout of electrochemiluminescence at the tip of an optical fiber
mercredi 11 mai 2016
H. Li, P. Garrigue, L. Bouffier, S. Arbault, A. Kuhn, N. Sojic Analyst, 2016, 141, 4299-4304 In this work, we report an original strategy for the wireless electrochemical generation of light at the tip of an optical fiber bundle, coupled with a simultaneous remote readout. An optical fiber bundle coated with a nanometer-thin gold film acts as a dual platform, on the one hand to locally... Read More...
Microscopic imaging and tuning of electrogenerated chemiluminescence with boron-doped diamond nanoelectrode arrays
mercredi 11 mai 2016
M. Sentic, F. Virgilio, A. Zanut, D. Manojlovic, S. Arbault, M. Tormen, N. Sojic, P. Ugo Anal. Bioanal. Chem., 2016, 408, 7085–7094 Nanoelectrode arrays (NEAs) are increasingly applied for a variety of electroanalytical applications; however, very few studies dealt with the use of NEAs as an electrochemical generator of electrogenerated chemiluminescence (ECL). In the present study, arrays... Read More...
Dual-color electrogenerated chemiluminescence from dispersions of conductive micro-beads addressed by bipolar electrochemistry
mercredi 11 mai 2016
A. de Poulpiquet, B. Diez-Buitrago, M. Milutinovic, B. Goudeau, L. Bouffier, S. Arbault, A. Kuhn, N. Sojic ChemElectroChem, 2016, 3, 404-409 Bipolar electrochemistry (BPE) is exploited here to address simultaneously thousands of carbon micro-beads dispersed in a solution. The suspension is placed in a capillary between 2 feeder electrodes generating in situ an electric field. The simultaneous... Read More...
Patchy colloidal particles for programmed self-assembly
vendredi 8 avril 2016
E. Duguet, C. Hubert, C. Chomette, A. Perro, S. Ravaine   C. R. Chimie. 2016, 19, 173  An increasing theoretical and experimental effort has been recently dedicated to the design and the fabrication of colloidal particles that may recognize each other and organize themselves via directional and specific interactions. These new colloidal particles exhibit a site-specific engineering of... Read More...
A Compelling Case for Bipolar Electrochemistry
vendredi 11 mars 2016
A. Kuhn, R. M. Crooks, S. Inagi ChemElectroChem 2016, 3, 351-352 Bipolar electrochemistry is a wireless way of carrying out redox reactions, which is currently undergoing a renaissance highlighted by the different contributions of this special issue. More information can be found in the Editorial by A. Kuhn, R. M. Crooks, and S. Inag.  
Electric fields for generating unconventional motion of small objects
jeudi 14 janvier 2016
L. Bouffier, V. Ravaine, N. Sojic and A. Kuhn.  Curr. Opin. Colloid Interface Sci., 2016, 21, 57-64. Electric fields can be used in several ways to generate motion of objects. In this article we focus on some more or less unconventional approaches, allowing in a direct or indirect way to design systems which show controlled motion, eventually combined with other functionalities such as light... Read More...
Selective Functionalization of Graphene Peripheries by using Bipolar Electrochemistry
lundi 11 janvier 2016
L. Zuccaro, A. Kuhn, M. Konuma, H. Ki Yu, K. Kern, K. Balasubramanian ChemElectroChem, 2016, 3, 372-377. We present a contactless strategy based on bipolar electrochemistry for the local chemical modification of monolayer graphene sheets supported on a substrate. Specifically, peripheral graphene regions are directly modified by copper nanoparticles, the characteristics of which are controllable... Read More...
Early Diagnosis of fungal Infections using piezomicrogravimetric and electric Chemosensors based on Polymers molecularly imprinted with D-arabitol
lundi 11 janvier 2016
M. Dabrowski, P. Sindhu Sharma, Z. Iskierko, K. Noworyta, M. Cieplak, S. Oborska, A. Kuhn, W. KutnerBiosensors and Bioelectronics, 2016, 79, 627-635. An elevated concentration of D-arabitol in urine, especially compared to that of L-arabitol or creatinine, is indicative of a fungal infection. For that purpose, we devised, fabricated, and tested chemical sensors determining D-arabitol. These... Read More...