E. Pedraza, A. Karajić, M. Raoux, R. Perrier, A. Pirog, F. Lebreton, S. Arbault, J. Gaitan, S. Renaud, A. Kuhn, J. Lang
Lab Chip, 2015, 15, 3880–3890
We are developing a cell-based bioelectronic glucose sensor that exploits the multi-parametric sensing
ability of pancreatic islet cells for the treatment of diabetes. These cells sense changes in the concentration
of glucose and physiological hormones and immediately react by generating electrical signals. In our sensor,
signals from multiple cells are recorded as field potentials by a micro-electrode array (MEA). Thus, cell
response to various factors can be assessed rapidly and with high throughput. However, signal quality and
consequently overall sensor performance rely critically on close cell–electrode proximity. Therefore, we
present here a non-invasive method of further exploiting the electrical properties of these cells to guide
them towards multiple micro-electrodes via electrophoresis. Parameters were optimized by measuring the
cell's zeta potential and modeling the electric field distribution. Clonal and primary mouse or human
β-cells migrated directly to target electrodes during the application of a 1 V potential between MEA
electrodes for 3 minutes. The morphology, insulin secretion, and electrophysiological characteristics were
not altered compared to controls. Thus, cell manipulation on standard MEAs was achieved without introducing
any external components and while maintaining the performance of the biosensor. Since the analysis
of the cells' electrical activity was performed in real time via on-chip recording and processing, this work
demonstrates that our biosensor is operational from the first step of electrically guiding cells to the final
step of automatic recognition. Our favorable results with pancreatic islets, which are highly sensitive and
fragile cells, are encouraging for the extension of this technique to other cell types and microarray devices.
Differential Photoluminescent and Electrochemiluminescent Behavior for Resonance Energy Transfer Processes in Thermoresponsive Microgels Monday, 25 July 2016
Florent Pinaud, Romain Millereux, Pierre Vialar-Trarieux, Bogdan Catargi, Sandra Pinet, Isabelle Gosse, Neso Sojic, Valérie Ravaine J. Phys. Chem. B, 2015, 119 (40), pp 12954–12961... Read More...
Electrogenerated Chemiluminescence of Cationic Triangulene Dyes: Crucial Influence of the Core Heteroatoms Thursday, 05 November 2015
C. Adam, A. Wallabregue, H. Li, J. Gouin, R. Vanel, S. Grass, J. Bosson, L. Bouffier, J. Lacour and N. Sojic. Chem. Eur. J., 2015, 21, 19243-19249. Trianguleniums are fascinating conjugated... Read More...
Guiding pancreatic beta-Cells to target Electrodes in a whole-cell Biosensor for Diabetes Friday, 25 September 2015
E. Pedraza, A. Karajić, M. Raoux, R. Perrier, A. Pirog, F. Lebreton, S. Arbault, J. Gaitan, S. Renaud, A. Kuhn, J. Lang Lab Chip, 2015, 15, 3880–3890 We are developing a cell-based bioelectronic...
The EChemPen: A Guiding Hand To Learn Electrochemical Surface Modifications Wednesday, 23 September 2015
M. Valetaud, G. Loget, J. Roche, N. Hüsken, Z. Fattah, V. Badets, O. Fontaine, D. Zigah. J. Chem. Educ., 2015, 92 (10), pp 1700–1704. The Electrochemical Pen (EChemPen) was developed as an... Read More...
Single-Step Screening of the Potential Dependence of Metal Layer Morphologies Along Bipolar Electrodes Monday, 07 September 2015
G. Tisserant, J. Gillion, J. Lannelongue, Z. Fattah, P. Garrigue, J. Roche, D. Zigah, B. Goudeau, A. Kuhn and L. Bouffier. ChemElectroChem, 2016, 3, 387-391. The preparation of surface... Read More...
Generation of Metal Composition Gradients by Means of Bipolar Electrodeposition Wednesday, 18 March 2015
G. Tisserant, Z. Fattah, C. Ayela, J. Roche, B. Plano, D. Zigah, B. Goudeau, A. Kuhn and L. Bouffier. Electrochim. Acta, 2015, 179, 276-281. Bipolar electrochemistry is an unconventional... Read More...
Effects of 50 Hz Magnetic Fields on Gap Junctional Intercellular Communication in NIH3T3 Cells Wednesday, 18 February 2015
Y. Percherancier, B. Goudeau, R. Charlet de Sauvage, F. Poulletier de Gannes, E. Haro, A. Hurtier, N. Sojic, I. Lagroye, S. Arbault, and B. Veyret Bioelectromagnetics, 2015, 36, 4, 287-293. The... Read More...
Synthesis of Multivalent Silica Nanoparticles combining both Enthalpic and Entropic Patchiness Friday, 16 January 2015
C. Hubert, C. Chomette, A. Désert, M. Sun, M. Treguer, S. Mornet, A. Perro, E. Duguet, S. Ravaine Faraday Discuss., 2015, 181, 139-146 Silica particles with a controlled number of entropic patches,... Read More...