Choix de langue :  flag-fr  flag-uk  
dimanche 23 avril 2017

Full-Spectral Multiplexing of Bioluminescence Resonance Energy Transfer in Three TRPV Channels

H. J. Ruigrok, G. Shahid, B. Goudeau, F. Poulletier de Gannes,E. Poque-Haro, A. Hurtier, I. Lagroye, P. Vacher, S. Arbault, N. Sojic, B. Veyret, Y. Percherancier

Biophysical Journal, 2017, 112, 87–98

Multiplexed bioluminescence resonance energy transfer (BRET) assays were developed tomonitor the activation of
several functional transient receptor potential (TRP) channels in live cells and in real time.We probed both TRPV1 intramolecular rearrangements and its interaction with Calmodulin (CaM) under activation by chemical agonists and temperature. OurBRET study also confirmed that: (1) capsaicin and heat promoted distinct transitions, independently coupled to channel gating, and that (2) TRPV1 and Ca2þ-bound CaM but not Ca2þ-free CaM were preassociated in resting live cells, while capsaicin activation induced both the formation of more TRPV1/CaM complexes and conformational changes. The BRET assay, based on the interaction with Calmodulin, was successfully extended to TRPV3 and TRPV4 channels. We therefore developed a full-spectral three-color
BRET assay for analyzing the specific activation of each of the three TRPV channels in a single sample. Such key improvement in BRET measurement paves the way for the simultaneous monitoring of independent biological pathways in live cells.

 

Capillary-assisted bipolar electrochemistry: a focused minireview
lundi 13 mars 2017
L. Bouffier, N. Sojic, A. Kuhn Electrophoresis, 2017, in press A bipolar electrode is quite unconventional as it behaves simultaneously as an anode and a cathode in contrast to a classic... Read More...
Hierarchical templating in deposition of semi-covalently imprinted inverse opal polythiophene film for femtomolar determination of human serum albumin
lundi 13 mars 2017
M. Dabrowski⁠, M. Cieplak⁠a⁠, P. Sindhu Sharma⁠, P. Borowicz⁠, K. Noworyta⁠, W. Lisowski⁠, F. D'Souza⁠⁠, A. Kuhn⁠, W. Kutner Biosens.&Bioelectron. 2017, 94, 155-161... Read More...
Spatially-resolved multicolor bipolar electrochemiluminescence
lundi 6 février 2017
Haidong Li, Laurent Bouffier, Stéphane Arbault, Alexander Kuhn, Conor F. Hogan, Neso Sojic Electrochemistry Communications, 2017, in press Here, we describe a new aspect of multicolor... Read More...
Co(III) Complexes of (1,3-Selen-2-yl)hydrazones and Their Sulphur Analogues: Comparative Structural, Electrochemical, Computational and Biological Activity Study
lundi 6 février 2017
Nenad R. Filipović, Hana Elshaflu, Sonja Grubišić, Ljiljana S. Jovanović, Marko Rodić, Irena Novaković, Aleksandar Malešević, Ivana S. Djordjević, Haidong Li, Nešo Šojić, Aleksandar... Read More...
Efficient Annihilation Electrochemiluminescence of Cationic Helicene Luminophores
vendredi 3 février 2017
Haidong Li, Silvia Voci, Antoine Wallabregue, Catherine Adam, Geraldine M. Labrador, Romain Duwald, Irene Hernández Delgado, Simon Pascal, Johann... Read More...
Applications of Electrogenerated Chemiluminescence in Analytical Chemistry
mercredi 18 janvier 2017
N. Sojic, S. Arbault, L. Bouffier, A. Kuhn Luminescence in Electrochemistry. Applications in Analytical Chemistry, Physics and Biology. Eds. F. Miomandre, P. Audebert, 2017. Springer. The great... Read More...
Recent Advances in Bipolar Electrochemistry
jeudi 12 janvier 2017
L. Bouffier, D. Zigah, N. Sojic, A. Kuhn Electroanalytical Chemistry: A Series of Advances, Volume 27, eds. A.J. Bard, C.G. Zoski, 2017, CRC Press, Taylor&Francis Group, in press The concept of... Read More...
Low-Molecular-Weight Hydrogels as New Supramolecular Materials for Bioelectrochemical Interfaces
jeudi 12 janvier 2017
D. Jain, A. Karajic, M. Murawska, B. Goudeau, S. Bichon, S. Gounel, N. Mano, A. Kuhn, P. Barthélémy ACS Appl. Mater. Interfaces 2017, in press, DOI: 10.1021/acsami.6b12890 Controlling the... Read More...
Highly Ordered Macroporous Electrodes
jeudi 12 janvier 2017
A. Kuhn, M. Heim Springer Handbook of Electrochemical Energy, eds. C. Breitkopf, K. Swider-Lyons, Chapter 6 p143-206, Springer Verlag, Berlin 2017, ISBN:978-3-662-46656-8 In this book chapter, we... Read More...
Full-Spectral Multiplexing of Bioluminescence Resonance Energy Transfer in Three TRPV Channels
jeudi 12 janvier 2017
H. J. Ruigrok, G. Shahid, B. Goudeau, F. Poulletier de Gannes,E. Poque-Haro, A. Hurtier, I. Lagroye, P. Vacher, S. Arbault, N. Sojic, B. Veyret, Y. Percherancier Biophysical Journal, 2017, 112,...
Bright Electrochemiluminescence Tunable in the Near-Infrared of Chiral Cationic Helicene Chromophores
mercredi 11 janvier 2017
Haidong Li, Antoine Wallabregue, Catherine Adam, Geraldine M. Labrador, Johann Bosson, Laurent Bouffier, Jérôme Lacour, Neso Sojic J. Phys. Chem. C,... Read More...
Scroll to top